Shared SOPs

You can only access our Standard Operating Procedures via VALIDATE Hub and each one linked below. If you need help getting access to the hub, email validate@ndm.ox.ac.uk.

• A Step-By-Step Beginner’s Protocol for Whole-Genome Sequencing of Human Bacterial Pathogen - Prepared by Sanjay S. Gautam
• Direct PBMC Mycobacterial Growth Inhibition Assay - Prepared by EURIPRED, Rachel Tanner
• Human Intracellular Cytokine Staining (ICS) Assay - Prepared by EURIPRED
• MIFlowCyt File - Prepared by Barbara Kronsteiner-Dobramysl
• PBMC (Peripheral Blood Mononuclear Cells) Isolation (PMB_SD_SOP_002) - Prepared by Barbara Kronsteiner-Dobramysl
• IFN-γ ELISpot (PMB_SD_SOP_003) - Prepared by Barbara Kronsteiner-Dobramysl
• Quick Hotshot DNA extraction protocol from mouse tissue - Prepared by Mirvat Surakhy
• Low-cost QPCR Method - Prepared by Federico Blanco, Fabiana Bigi
• Low-cost method for RNA purification from PBMC - Prepared by Federico Blanco, Fabiana Bigi