Shared SOPs

VALIDATE members can access our Standard Operating Procedures via the VALIDATE Hub by clicking the relevant link below. If you need help getting access to the hub, email validate@ndm.ox.ac.uk.

• A Step-By-Step Beginner’s Protocol for Whole-Genome Sequencing of Human Bacterial Pathogen - Prepared by Sanjay S. Gautam
• Direct PBMC Mycobacterial Growth Inhibition Assay - Prepared by EURIPRED, Rachel Tanner
• Human Intracellular Cytokine Staining (ICS) Assay - Prepared by EURIPRED
• MIFlowCyt File - Prepared by Barbara Kronsteiner-Dobramysl
• PBMC (Peripheral Blood Mononuclear Cells) Isolation (PMB_SD_SOP_002) - Prepared by Barbara Kronsteiner-Dobramysl
• IFN-γ ELISpot (PMB_SD_SOP_003) - Prepared by Barbara Kronsteiner-Dobramysl
• Quick Hotshot DNA extraction protocol from mouse tissue - Prepared by Mirvat Surakhy
• Low-cost QPCR Method - Prepared by Federico Blanco, Fabiana Bigi
• Low-cost method for RNA purification from PBMC - Prepared by Federico Blanco, Fabiana Bigi