The group of Eva Gluenz studies study gene function in the protozoan parasite Leishmania to dissect and understand the cellular mechanisms that enable these unicellular parasites to pass through their life cycle and cause disease in humans and animals. The group is located at the Institute of Cell Biology, within the Faculty of Science of the University of Bern.
We are recruiting two Research Assistants to support the generation of CRISPR-Cas9 gene deletion cell lines for a genome-wide phenotyping screen of the protozoan parasite Leishmania. This is part of a Welcome Trust funded collaborative project (LeishGEM.org), which has two genome-wide reverse genetics screens as a foundation, followed up with cutting-edge molecular biology, cell biology and data science to define the most important pathways for pathogenicity.
The purpose of these Research Assistant posts is to generate libraries of CRISPR knockout mutants and study their phenotypes in collaboration with other members of the group.
• Maintaining cell cultures
• Generation and characterisation of gene-edited cell lines
• Generation of Illumina sequencing libraries
• Recording data
• Contribute to presentation of results
• Perform administrative tasks related to the research project
• Be responsible for maintenance of equipment and user training
• Academic degree in a relevant subject
• Experience in molecular biology including PCR, cloning and sequencing
• Experience of cell culture and sterile technique in the laboratory
• (For one of the two posts) experience with NGS library preparation
• IT and data analysis skills
• Fluent in English
• Proven ability to work independently
• Research Assistant positions for 4 years with option of extension
• A stimulating, cooperative, and supportive environment and close collaboration with international research teams
• Multiple opportunities for continuous education