Central University of Rajasthan, India
M. tuberculosis, Intracellular pathogens, Vaccine, Drug Target, Toxin-Antitoxin systems
I have worked predominantly on the “Identification and Characterization of promoters for Toxin-Antitoxin loci of Mycobacterium tuberculosis” and has done some significant work in the domain of expression of mycobacterial proteins & cloning and expression of mycobacterial sigma factors. I have developed an EGFP reporter vector system and it was used to identify and characterize promoters of Toxin-Antitoxin loci encoded by Mycobacterium tuberculosis. I have also developed a promoter reporter vector system based on selection and optimized it for identification of genome wide promoters of Mycobacterium tuberculosis. Along with this, I also worked to construct two whole genome libraries of Mycobacterium tuberculosis, and selected the promoters followed by mapping of promoters for genome-wide toxin antitoxin loci using next generation sequencing technology promoters as a part of her PhD work. This work adds new dimension to understand the regulatory mechanisms that lead to differential activation of these Toxin Antitoxin loci in M. tuberculosis and adds a lot of valuable information to the existing literature. I have handled multiple projects at a time and has good teamwork capability.
Currently, I am focusing on the vaccine development, drug target and drug repurposing for human infectious diseases. Additionally, my research work also deals with identification and characterization of Toxin-Antitoxin loci of human pathogen Helicobacter pylori.
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University of York Research Database
Gupta N, Khatoon N, Verma VK, Prajapati VK; (2019), Structural vaccinology approach to investigate the virulent and secretory proteins of Bacillus anthracis for devising Anthrax next-generation vaccine. J Biomol Struct Dyn., Doi 10.1080/07391102.2019.1688197.
Gupta N, Maurya S, Verma H, Verma VK; (2019), Unravelling the factors and mechanism involved in persistence: host pathogen interaction in Helicobacter pylori. J Cell Biochem, 120(11), 18572-18587.
Deshmukh AS, Agarwal M, Mehra P, Gupta A, Gupta N, Doerig CD, Dhar SK; (2015), Regulation of Plasmodium falciparum Origin Recognition Complex subunit 1 (PfORC1) function through phosphorylation mediated by CDK-like kinase PK5. Molecular Microbiology, 98(1), 2015, 17-33.
Venkataraman B, Gupta N, Gupta A; (2013), A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis. J Microbiol Methods, 93(3), 198-202.
Nidhi Gupta & Vijay K. Verma; (2019), Next Generation Sequencing and its application: empowering in public health beyond reality” in the book entitled “Microbial technology for the welfare of society” edited by Dr. Pankaj Arora in Springer eBook ISBN: 978-981-13-8844-6.