Vijay Verma

Assistant Professor
UDSC, India
Email: vijayscmmjnu@gmail.com
VALIDATE Role:
Network Associate
Research Keywords:
Intracellular pathogens, Vaccine, Drug Target, Toxin-Antitoxin systems
Biography:
I have worked predominantly on the “Identification and Characterization of promoters for Toxin-Antitoxin loci of Mycobacterium tuberculosis” and has done some significant work in the domain of expression of mycobacterial proteins & cloning and expression of mycobacterial sigma factors. I have developed an EGFP reporter vector system and it was used to identify and characterize promoters of Toxin-Antitoxin loci encoded by Mycobacterium tuberculosis. I have also developed a promoter reporter vector system based on selection and optimized it for identification of genome wide promoters of Mycobacterium tuberculosis. Along with this, I also worked to construct two whole genome libraries of Mycobacterium tuberculosis, and selected the promoters followed by mapping of promoters for genome-wide toxin antitoxin loci using next generation sequencing technology promoters as a part of her PhD work. This work adds new dimension to understand the regulatory mechanisms that lead to differential activation of these Toxin Antitoxin loci in M. tuberculosis and adds a lot of valuable information to the existing literature. I have handled multiple projects at a time and has good teamwork capability.
Currently, I am focusing on the vaccine development, drug target and drug repurposing for human infectious diseases. Additionally, my research work also deals with identification and characterization of Toxin-Antitoxin loci of human pathogen Helicobacter pylori.
Key Publications:
- Gupta N, Behera DK, Prajapati VK, Verma VK. (2022) A comprehensive approach to discover Toxin-Antitoxin systems from human pathogen Helicobacter pylori: A poison and its antidote encapsulated in the genome. Life Sciences. 2022 1;288:120149. doi: 10.1016/j.lfs.2021.120149. (IF-6.78, ISSN: 0024-3205) (1st Jan 2022). (*Corresponding author) (URL: https://doi.org/10.1016/j.lfs.2021.120149
- Ojha R, Gupta N, Naik B, Singh S, Verma VK, Prusty D, Prajapati VK (2020) High throughput and comprehensive approach to develop multiepitope vaccine against minacious COVID-19. European Journal of Pharmaceutical Sciences, 151(2020) 105375. doi:10.1016/j.ejps.2020.105375. (IF-5.11, ISSN: 0928-0987) (14th May 2020). URL: https://doi.org/10.1016/j.ejps.2020.105375
- Gupta N, Regar H, Verma VK, Prusty D, Mishra A, Prajapati VK; (2020), Receptor-ligand based molecular interaction to discover adjuvant for immune cell TLRs to develop next-generation vaccine. International Journal of Biological Macromolecules, 152, 535-545. (IF- 8.0 ; ISSN: 0141-8130) (1st June 2020). URL: https://doi.org/10.1016/j.ijbiomac.2020.02.297
- Gupta, N., Khatoon, N., Mishra, A., Verma, VK., and Prajapati, V.K.; (2019), Structural vaccinology approach to investigate the virulent and secretory proteins of Bacillus anthracis for devising Anthrax next-generation vaccine. Journal of Biomolecular Structure and Dynamics. 38(16):4895-4905. (IF- 5.2, ISSN: 0739-1102) (18th Nov 2019) URL: https://doi.org/10.1080/07391102.2019.1688197
- Gupta, N; Maurya, S; Verma, H and Verma, VK* (2019) Unravelling the factors and mechanism involved in persistence: host-pathogen interactions in Helicobacter pylori. Journal of Cellular Biochemistry. 120(11):18572-18587 (*Corresponding author) (IF- 4.48, ISSN:1097-4644) (25th June 2019) URL: https://doi.org/10.1002/jcb.29201
- Mohammed, K; Dubey, P; Verma, V; Dasgupta, S and Dhar, SK (2018) Helicobacter pylori shows asymmetric and polar cell divisome assembly associated with DNA replisome. FEBS J. 285(13):2531-2547. (IF-5.6, ISSN: 1742-464X ) (9th May 2018), URL: https://doi.org/10.1111/febs.14499
- Pandey, P; Verma, V; Kumari, N; Dhar, SK and Gourinath, S. (2017) Targeting β-clamp in Helicobacter pylori with FDA approved drugs yields micromolar inhibition by diflunisal. FEBS Letter 591(15):2311-2322 (IF-3.86, ISSN: 1873-3468) (28th June 2017) URL: https://doi.org/10.1002/1873-3468.12734
- Verma, V; Kumar, A; Nitharwal, RG; Alam, J; Mukhopadhyay, A; Dasgupta, S and Dhar SK; (2016) Modulation of the enzymatic activities of replicative helicase (DnaB) by interaction with Hp0897: A possible mechanism for helicase loading in Helicobacter pylori. Nucleic Acids Research 44(7):3288-3303. (IF-19.16, ISSN: 0305-1048), (21st March 2016( URL: https://doi.org/10.1093/nar/gkw148
- Abdul Rehman, SA*; Verma, V*; Mazumder, M; Dhar, SK and Gourinath, S (2013) Crystal structure and mode of helicase binding of the C-terminal domain of primase from Helicobacter pylori. J. Bacteriol 195(12):2826-2838. (*Equally contributed). (IF-3.47, ISSN: 0021-9193), (24th May 2013) URL: https://doi.org/10.1128/JB.00091-13
- Nitharwal, RG*; Verma, V*; N. Rao, S; Dasgupta, S; Choudhury, NR and Dhar, SK (2011) DNA binding activity of Helicobacter pylori DnaB helicase: the role of N-terminal domain in modulating DNA binding activities. FEBS J. 279(2): 234-50. (*Equally contributed); (Figure ‘5C’ as cover page). (IF-5.6, ISSN: 1742-464X), (10th Nov 2011), URL: https://doi.org/10.1111/j.1742-4658.2011.08418.x