Wynand Johan Goosen
Postdoctoral Research Fellow
Stellenbosch University, South Africa
Tel: (+27) 21 938 9073
M. tuberculosis, M. bovis, domestic animals, African wildlife, diagnostics, One Health.
In 2012, Wynand Goosen joined the Department of Biomedical Sciences as an honour student under the supervision of the head of department Prof Paul van Helden and Dr Sven Parsons. During his honour’s year, Wynand developed an in vitro interferon-gamma (IFN-γ) protein release assay and an IFN-γ gene expression assay by adapting the QuantiFERON-TB Gold (InTube) assay for the diagnosis of Mycobacterium bovis in African Wild dogs.
Thereafter, Wynand pursued an MSc degree with the same group, which he subsequently upgraded to a PhD. During this time, Wynand’s focus shifted to characterizing and utilizing the immune responses of African buffaloes and cattle in order to diagnose M. bovis infection. During this time, Wynand published various first author and co-author research articles in international peer-reviewed journals and subsequently graduated with a PhD December 2016.
In 2017, Wynand decided to pursue a year of clinical tuberculosis research experience at University of Cape Town’s Lung Infection and Immunity Unit. During this time, Wynand learned a lot about designing and developing innovative molecular diagnostic tools for the diagnosis of drug-resistant tuberculosis. He was a part of various therapeutic intervention projects surrounding the treatment of extensive drug resistant TB infected patients and co-designed various devices able to measure the infectiousness of these patients in their community. With this knowledge and experience, returned to the group and started a postdoctoral research fellowship in 2018. Wynand quickly initiated various projects and collaborations with companies focussing on rapid ante-mortem and post-mortem detection (DNA detection and enhanced culture) of Tuberculosis in African elephants and rhinoceros using unconventional difficult to culture samples such as trunk wash and bronchial alveolar lavage (BAL) samples. Collaborations with Cepheid and TiKa Diagnostics led to the evaluation of a human GeneXpert MTB/RIF Ultra assay (Ultra) and the new TiKa-MGIT culture test, respectively. Until now, we have shown that Ultra is able to detect TB bacilli as low as two CFU/ml and the new TiKa-MGIT system to drastically enhance mycobacterial culture from paucibacillary samples. Furthermore, this novel culture method not only enhances the diagnosis of TB, but also provides a live TB culture enabling downstream typing and better epidemiological studies. These successful collaborations lead to Wynand being awarded a spot in the top 10 young emerging scientists in South Africa selected by the National Science and Technology Forum and his inauguration into the National Geographic Society as an explorer.
Wynand truly believe his innovative research will aid veterinarians to make faster, more informed decisions on animal tuberculosis management, enhance the efficacy of control measures aimed at reducing diseases spread and help rationalisation of efforts to eradicate TB in South Africa.
Profile page on ResearchGate
Goosen, W.J., Cooper, D., Miller, M.A., van Helden, P.D., Parsons, S.D.C., 2015. IP-10 Is a sensitive biomarker of antigen recognition in whole-blood stimulation assays used for the diagnosis of Mycobacterium bovis infection in African Buffaloes (Syncerus caffer). Clin. Vaccine Immunol. CVI 22, 974 978.
Goosen, W.J., Cooper, D., Warren, R.M., Miller, M.A., van Helden, P.D., Parsons, S.D.C., 2014a. The evaluation of candidate biomarkers of cell-mediated immunity for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer). Vet. Immunol. Immunopathol. 162, 198 202.
Goosen, W.J., Miller, M.A., Chegou, N.N., Cooper, D., Warren, R.M., van Helden, P.D., Parsons, S.D.C., 2014b. Agreement between assays of cell-mediated immunity utilizing Mycobacterium bovis specific antigens for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer). Vet. Immunol. Immunopathol. 160, 133 138.
Goosen, W.J., van Helden, P.D., Warren, R.M., Miller, M.A., Parsons, S.D.C., 2016. The stability of plasma IP-10 enhances its utility for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer). Vet. Immunol. Immunopathol. 173, 17 20.
Goosen, W.J., Kerr, T.J., Kleynhans, L., Warren, R.M., van Helden, P.D., Persing, D.H., Parsons, S.D.C., Buss, P., Miller, M.A., 2019. The GeneXpert MTB/RIF Ultra assay detects Mycobacterium tuberculosis complex DNA in white rhinoceros (Ceratotherium simum) and African elephants (Loxodonta africana). Sci. Rep.
Goosen, W.J., Kerr, T.J., Kleynhans, L., Warren, R.M., van Helden, P.D., Björn, S., Parsons, S.D.C., Buss, P., Miller, M.A., 2019. The VetMAXTM M. tuberculosis Complex PCR Kit detects MTBC DNA in antemortem and postmortem samples from white rhinoceros (Ceratotherium simum), African elephants (Loxodonta africana) and African buffaloes (Syncerus caffer). Vet. Immunol. Immunopathol.
Meiring, C., van Helden, P.D., Goosen, W.J., 2018. TB Control in Humans and Animals in South Africa: A Perspective on Problems and Successes. Front. Vet. Sci. 5.
Miller, M.A., Buss, P., Parsons, S.D.C., Roos, E., Chileshe, J., Goosen, W.J., van Schalkwyk, L., de Klerk-Lorist, L.-M., Hofmeyr, M., Hausler, G., Rossouw, L., Manamela, T., Mitchell, E.P., Warren, R., van Helden, P., 2018. Conservation of White Rhinoceroses Threatened by Bovine Tuberculosis, South Africa, 2016 2017. Emerg. Infect. Dis. 24, 2373 2375.