Suzie Madaki Poster 2023

Dr Suzie Madaki

Bayero University, Kano, Nigeria

An Assessment Of Percentage and Absolute Cd4 Lymphocytes Levels and Factors Influencing Their Levels in Tuberculosis Patients Receiving Treatment n Barau Dikko Teaching Hospital, Kaduna, Nigeria.

Poster Abstract

Background: The role of CD4 T cells in immunity against tuberculosis has been widely acknowledged. This study determined CD4 percentage and absolute levels in tuberculosis patients undergoing tuberculosis treatment and compared these levels with the presence of some factors which could modify the quantities of CD4 parameters in tuberculosis patients.

Methods: Multicolour fluorescence imaging and absorbance spectrometry microscopy were used to enumerate CD4 absolute count, CD4 percentage and haemoglobin concentration in serum from ninety tuberculosis patients receiving treatment.

Questionnaires and interviews were used to determine factors like BCG scar (evidence of vaccination), vitamin A supplementation, previous infection, awareness of zoonotic TB, malnutrition and drug resistance.

Results:

  • Absolute CD4 count significantly differed between 51 males (mean age: 33.5) and 39 females (mean age: 32.2) at 5% level of significance. 37 (41.1%) had BCG scar, 53(58.9%) did not have.
  • T-test indicated no significant difference in CD4 parameters as compared with BCG vaccination, BCG scar, knowledge of zoonotic diseases, knowledge of zoonotic tuberculosis and with vitamin A supplementation.
  • Conversely, there was significant difference between mean age of males and females and in the CD4 counts and absolute CD4 count when compared with maternal BCG vaccination, and maternal immunization (vaccination Scar).
  • Significant difference in CD4 counts compared with malnutrition. Significant difference in the absolute CD4 counts compared with drug resistance. Chi-square analysis revealed significant association between vitamin A supplementation and BCG scar. Pearson correlation coefficient indicated weak negative relationship between CD4%, absolute CD4 and age, weak positive relationship between HB and age.
  • All at a 5% level of significance. Conclusion: Malnutrition, drug resistance, maternal immunization, and age influence levels of CD4 in tuberculosis patients.

 

Biography

I am a PhD student of Medical Microbiology, emphasis on immunology. My research focus is on tuberculosis, Single Nucleotide Polymorphism, cytokines and drug resistance in humans. I am now collecting blood and sputum samples at TB DOTS clinics and National Tuberculosis and Leprosy Training Centre and Referral Hospital, Saye Village, Zaria, Kaduna State, and administering a questionnaire to collect information on awareness of zoonotic diseases and possible exposure to zoonotic tuberculosis. I hope to publish my thesis 'DETERMINATION OF INF-γ and IL-10 SINGLE NUCLEOTIDE POLYMORPHISM AMONG DRUG RESISTANT TUBERCULOSIS PATIENTS IN KADUNA STATE' when all samples have been collected and all analysis done.

I have also worked on CD4 levels in tuberculosis patients and the factors which influence levels of absolute CD4 count, percent CD4 and haemoglobin levels in tuberculosis patients.

A graduate of Veterinary Medicine from Ahmadu Bello University, Zaria with MSc Medical & Poisonous Plants studies and Post Graduate Diploma in GIS. Also attended several professional/short courses: Human and Veterinary Vaccinology, Oxford University, UK; Control Strategies for Communicable and Non-communicable Diseases, KIT Royal Tropical Institute, Amsterdam, Netherlands; Sustainable diets, Humboldt University, Berlin, Germany; WASH and Disease Surveillance and Immunization, Bayero University, Kano, Nigeria.

As a specialist on animal health and zoonotic diseases with the Kaduna state Agricultural Development Agency, I coordinate awareness creation and health education for livestock farmers, pet breeders and owners, veterinary drug vendors and communities on animal and zoonotic diseases. I currently also working as a programme manager, supporting the management of interventions targeting control, elimination and eradication of neglected tropical diseases in Nigeria. With previous experience under the Polio and Routine Immunization program to improve uptake of immunization services. I coordinate, manage and lead the design and delivery of projects and interventions aimed at improving public health.