Innovative tools to measure mycobacterial antigen expression in tissue

VALIDATE Pump-Priming Project - January 2020 to July 2021

Led by:
Dr Simon Waddell; VALIDATE Investigator; University of Sussex, UK

With:
Dr Javier Salguero Bodes; VALIDATE Investigator; PHE, UK
Prof Gobena Ameni; VALIDATE Associate; Addis Ababa University, Ethiopia
Dr Sally Sharpe; VALIDATE Investigator; PHE, UK
Dr Ann Rawkins; VALIDATE Investigator; PHE, UK
 

Project Aims

Before we can hope to eradicate tuberculosis suffering worldwide, we need to identify the key protective antigens to target for vaccination that will eliminate Mycobacterium tuberculosis from the lung. This project will develop new RNA extraction and profiling techniques that will allow us to define the expression of M. tuberculosis (M.tb) antigens in lung tissue, at the site of disease. Our unique approach, building on international expertise from the UK and Ethiopia, will identify the key M.tb antigens for novel vaccine development, offering new opportunities for transformational change.

Project Outcomes

This award enabled us to develop key techniques to measure Mycobacterium tuberculosis gene expression in fresh and FFPE (formalin-fixed paraffin-embedded) tissue. This will allow us to characterise previously intractable host-pathogen interactions at the site of disease. We have also built a strong UK and international collaborative team to further extend this research.

Project Outputs

• Medley J, Goff A, Bettencourt PJG, Dare M, Cole L, Cantillon D, Waddell SJ (2022). Dissecting the Mycobacterium bovis BCG response to macrophage infection to help prioritise targets for anti-tuberculosis drug and vaccine discovery. Vaccines (Basel); 10(1):113. PMID: 35062774. PMCID: PMC8780277